{"id":9953,"date":"2023-01-26T14:49:14","date_gmt":"2023-01-26T14:49:14","guid":{"rendered":"https:\/\/targetmalaria.org\/biologie-moleculaire\/"},"modified":"2025-11-17T14:55:35","modified_gmt":"2025-11-17T14:55:35","slug":"biologie-moleculaire","status":"publish","type":"page","link":"https:\/\/targetmalaria.org\/fr\/notre-mission\/science\/biologie-moleculaire\/","title":{"rendered":"Biologie mol\u00e9culaire"},"content":{"rendered":"
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Nos \u00e9quipes de biologie mol\u00e9culaire se concentrent sur le d\u00e9veloppement des premi\u00e8res \u00e9tapes de la technologie d\u2019impulsion g\u00e9n\u00e9tique.<\/h2>\n<\/div>\n\n\n\n
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Nous commen\u00e7ons par un processus de d\u00e9couverte et de compr\u00e9hension du type de modifications g\u00e9n\u00e9tiques que nous devons introduire dans le g\u00e9nome du moustique pour g\u00e9n\u00e9rer l\u2019effet d\u00e9sir\u00e9. Nous \u00e9tudions par exemple quels g\u00e8nes sont importants pour la reproduction du moustique et ce qui se passe lorsque ces g\u00e8nes sont d\u00e9sactiv\u00e9s.<\/p>\n\n\n\n

Nous utilisons ensuite une technologie de pointe pour \u00e9diter ces g\u00e8nes en laboratoire, en utilisant notamment CRISPR\/Cas9, et \u00e9tudier l\u2019effet de la modification sur le moustique modifi\u00e9. \u00c0 l\u2019aide d\u2019outils modernes de biologie mol\u00e9culaire, nous assemblons les composants mol\u00e9culaires n\u00e9cessaires \u00e0 la construction d\u2019une impulsion g\u00e9n\u00e9tique en laboratoire, et nous l\u2019introduisons tr\u00e8s sp\u00e9cifiquement dans le g\u00e9nome du moustique. Cela peut se faire entre autres en coupant des s\u00e9quences d\u2019ADN sp\u00e9cifiques du g\u00e8ne que nous voulons modifier.<\/p>\n\n\n\n

Comment nous g\u00e9n\u00e9rons des moustiques g\u00e9n\u00e9tiquement modifi\u00e9s<\/h3>\n\n\n\n

Pour g\u00e9n\u00e9rer des moustiques g\u00e9n\u00e9tiquement modifi\u00e9s, nous assemblons d\u2019abord en laboratoire les composants mol\u00e9culaires et l\u2019ADN que nous voulons introduire dans le g\u00e9nome du moustique. Au microscope, \u00e0 l\u2019aide d\u2019aiguilles tr\u00e8s fines, nous injectons l\u2019ADN dans les embryons de moustiques d\u00e8s les premi\u00e8res heures suivant la ponte. Nous devons effectuer ces injections sur des centaines ou des milliers d\u2019embryons individuels pour g\u00e9n\u00e9rer un seul type de modification.<\/p>\n\n\n\n

Avec l\u2019ADN du g\u00e8ne d\u2019int\u00e9r\u00eat, nous introduisons \u00e9galement le g\u00e8ne d\u2019un marqueur fluorescent qui fera briller le moustique sous un microscope \u00e0 fluorescence. Ceci nous permet d\u2019identifier les moustiques modifi\u00e9s. Le s\u00e9quen\u00e7age des g\u00e8nes ou d\u2019autres techniques, telles que la PCR, sont \u00e9galement utilis\u00e9s pour v\u00e9rifier que la modification a r\u00e9ussi.<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Nos \u00e9quipes de biologie mol\u00e9culaire se concentrent sur le d\u00e9veloppement des premi\u00e8res \u00e9tapes de la technologie d\u2019impulsion g\u00e9n\u00e9tique. Nous commen\u00e7ons par un processus de d\u00e9couverte et de compr\u00e9hension du type de modifications g\u00e9n\u00e9tiques que nous devons introduire dans le g\u00e9nome du moustique pour g\u00e9n\u00e9rer l\u2019effet d\u00e9sir\u00e9. Nous \u00e9tudions par exemple quels g\u00e8nes sont importants pour […]<\/p>\n","protected":false},"author":31,"featured_media":18841,"parent":10966,"menu_order":26,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_relevanssi_hide_post":"","_relevanssi_hide_content":"","_relevanssi_pin_for_all":"","_relevanssi_pin_keywords":"","_relevanssi_unpin_keywords":"","_relevanssi_related_keywords":"","_relevanssi_related_include_ids":"","_relevanssi_related_exclude_ids":"","_relevanssi_related_no_append":"","_relevanssi_related_not_related":"","_relevanssi_related_posts":"","_relevanssi_noindex_reason":"","featured_image_focal_point":[],"footnotes":""},"class_list":["post-9953","page","type-page","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"\nBiologie mol\u00e9culaire - Target Malaria<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/targetmalaria.org\/fr\/notre-mission\/science\/biologie-moleculaire\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Biologie mol\u00e9culaire - Target Malaria\" \/>\n<meta property=\"og:description\" content=\"Nos \u00e9quipes de biologie mol\u00e9culaire se concentrent sur le d\u00e9veloppement des premi\u00e8res \u00e9tapes de la technologie d\u2019impulsion g\u00e9n\u00e9tique. 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